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Impact of nitrogen fertilization and drought on glutenins content in winter wheat
 Vostrejš, Pavel ; Vítová, Eva (referee) ; Hrstka, Miroslav (advisor)
In this bachelor thesis the effects of nitrogen fertilization and drought stress on glutenin fraction of winter wheat (Triticum aestitivum L.) were studied. Winter wheat cultivar Tiguan was grown under different rainfall mode, on the one hand in drought (DRY), on the other hand in normal downfall (WET). Part of the samples was treated with nitrogen fertilization at a dose of 140 kg / ha (N140), the other part remained without fertilization (N0). After the gliadins were separated, the glutenins from the samples were extracted with 50% isopropanol with 1% DTT. Individual glutenin fractions were separated by SDS-PAGE and quantified by computer densitometry. The drought did not have a statistically significant effect on any of the glutenin subunits. Nitrogen fertilization caused an increase in the total amount of glutenin as well as the amount of individual subunits. Interaction of water regime treatment and fertilization has not led to an increase in glutenin levels in HMW 3 GS and LMW 5 GS in DRY conditions.
Detailed record
Impact of nitrogen fertilization and drought on glutenins content in winter wheat
 Vostrejš, Pavel ; Vítová, Eva (referee) ; Hrstka, Miroslav (advisor)
In this bachelor thesis the effects of nitrogen fertilization and drought stress on glutenin fraction of winter wheat (Triticum aestitivum L.) were studied. Winter wheat cultivar Tiguan was grown under different rainfall mode, on the one hand in drought (DRY), on the other hand in normal downfall (WET). Part of the samples was treated with nitrogen fertilization at a dose of 140 kg / ha (N140), the other part remained without fertilization (N0). After the gliadins were separated, the glutenins from the samples were extracted with 50% isopropanol with 1% DTT. Individual glutenin fractions were separated by SDS-PAGE and quantified by computer densitometry. The drought did not have a statistically significant effect on any of the glutenin subunits. Nitrogen fertilization caused an increase in the total amount of glutenin as well as the amount of individual subunits. Interaction of water regime treatment and fertilization has not led to an increase in glutenin levels in HMW 3 GS and LMW 5 GS in DRY conditions.
Detailed record

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